Do Differentiated Osteoblasts and Allogeneic Mesenchymal Stromal Cells Augment Bone Formation?

Introduction: Fixation of massive bone tumour endoprosthetic replacements is not as successful as conventional joint replacement surgery and aseptic loosening is the major cause of implant failure. Increased bone growth and contact to the implan shaft by bony bridging has been shown vital in reducing implant failure. Our previous work has shown that spraying autogenic bone marrow derived mesenchymal stromal cells (MSCs) within fibrin glue onto the grooved hydroxyapatite (HA) collars of segmental bone tumour implants significantly increased bone formation in an ovine model1. In this study we investigated the hypothesis that allogeneic MSCs and MSC’s differentiated in an osteogenic supplemented media (ogMSCs) will improve bone growth equal to undifferentiated autogenic MSCs in an in vivo massive tumor implant ovine model. This study also hypothesized that bone growth is dependent on cell concentration.